Part:BBa_K1640020

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Overview

This biobrick contains one gene from the photosystem II complex of Chlamydomonas reinhardtii - psbA.

- psbA encodes D1, a subunit of the Photosystem II reaction centre.

This part was designed in conjunction with BBa_K1640024, and together they form operon 4 in our set of photosystem II operons:

Biology & Literature

PsbA encodes the Photosystem II reaction centre subunit D1. This product forms a heterodimer with D2, encoded by psbD, to form the Photosystem II reaction centre (Marder, Chapman, Telfer, Nixon, & Barber, 1987). The primary function of the D1/D2 reaction centre complex is to transfer electrons from the chlorophyll P680 to two quinones, designated QA and QB. To this end, D1 has functions both at the oxidation and reduction stages of the reaction.

D1 accepts electrons from P680, transferring it to QB. A tyrosine of D1 reduces the resulting P680 cation, resulting in a tyrosine radical. This radical acts as an oxidising agent for the oxidation of water through the oxygen evolving complex (OEC) of PSII (Ferreira, Iverson, Maghlaoui, Barber, & Iwata, 2004). In addition, this protein is vital to the assembly of both the OEC and QB within PSII. Site-directed mutagenesis studies have revealed an aspartate residue of D1 to be both structurally coupled to and involved in the assembly of the Mn cluster within the OEC (Chu, Debus, & Babcock, 2001; Nixon & Diner, 1992). Similarly, inhibition studies involving the binding of herbicides to D1 have revealed this protein to contain a niche for the binding of QB (Erickson et al., 1989; Jansen, Mattoo, Malkin, & Edelman, 1993).

Protein information

psbA

mass: 50.92kDa

sequence: METLFNGTLTVGGRDQETTGFAWWSGNARLINLSGKLLGAHVAHAGLIVFWAGAMNLFEVSHFVPEKPMYEQGLILLPHIATLGYGVGPGGEIIDTFPYFVSGVLHLISSAVLGFGGVYHSLIGPETLEESYPFFGYVWKDKNKMTNILGYHLIMLGLGAWLLVWKAMYFGGVYDTWAPGGGDVRVITNPTTNAAVIFGYLVKSPFGGDGWICSVDNMEDIIGGHIWIGTLEILGGIWHIYTTPWPWARRAFVWSGEAYLSYSLGAIGVMGFIACCMSWFNNTAYPSEFYGPTGPEASQSQAFTFLVRDQRLGANVASAQGPTGLGKYLMRSPTGEIIFGGETMRFWDFRGPWLEPLRGPNGLDLNKLKNDIQPWQERRAAEYMTHAPLGSLNSVGGVATEINAVNFVSPRSWLACSHFCLGFFFFIGHLWHAGRARAAAAGFEKGIDRFDEPVLSMRPLD*