Composite

Part:BBa_K1684003

Designed by: Juan Jos Quispe Haro   Group: iGEM15_BIOSINT_Mexico   (2015-09-17)
Revision as of 08:09, 18 September 2015 by JuanjoQuispe (Talk | contribs) (→‎Results)

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RBS + amilGFP

Yellow chromoprotein optimized for E.coli K12 with the standar RBS

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.

This version with the standar RBS is codon optimized for the expression in E.coli K12.

Results

The Figure 1 present different samples of parts used for transformation of competent cells composed by a RBS (BBa_B0034) and the optimized chromoprotein sequence amilGFP (BBa_K1684001 ).

The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 5 and Line 6 contain duplicate BBa_K1684003 (BBa_B0034 + BBa_K1684001) biobrick; however, a positive result is observed only in Line 5, with size of 2822 bp, while in line 6 only presents contamination, without the presence of any genetic material.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680


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