Part:BBa_K1813025

CYP6G1 Expression Cassette with LacI Reversed

A construct used to achieve the bacterial expression of CYP6G1 (Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 105
  Illegal BglII site found at 1737
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2327
  
• 1000
  COMPATIBLE WITH RFC[1000]

Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6G1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.