Coding

Part:BBa_K1789008:Design

Designed by: Xinyuan Qiu   Group: iGEM15_NUDT_CHINA   (2015-09-14)
Revision as of 07:46, 18 September 2015 by Qiuxinyuan12 (Talk | contribs)

TALE1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1075


Design Notes

This part is designed based on the sequence of BM1 in our project.

NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard.

TALE1.jpg Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids


The protocol can be reached in our wiki


Source

This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves.

References