Composite
Part:BBa_K1795001
Designed by: Andrew D Halleran, Joseph L Maniaci Group: iGEM15_William_and_Mary (2015-09-17)
E. Coli Codon Optimized fdCAS9 under R0010
This sequence codes for dCas9 and is codon optimized for E. Coli. It is under the promoter R0010 and has RBS B0034 and DT B0015.
When co-transformed with RFP and BBa_K1795002 97% reduction of RFP was observed. File:WMGibsonIconColor.png Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |