Composite

Part:BBa_K1795001

Designed by: Andrew D Halleran, Joseph L Maniaci   Group: iGEM15_William_and_Mary   (2015-09-17)
Revision as of 07:12, 18 September 2015 by Cgolino (Talk | contribs)

E. Coli Codon Optimized fdCAS9 under R0010

This sequence codes for dCas9 and is codon optimized for E. Coli. It is under the promoter R0010 and has RBS B0034 and DT B0015.

When co-transformed with RFP and BBa_K1795002 97% reduction of RFP was observed. File:WMGibsonIconColor.png Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


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