Composite

Part:BBa_K1804001:Design

Designed by: Wong Chi Yan   Group: iGEM15_SPSingapore   (2015-09-17)
Revision as of 06:37, 18 September 2015 by Wongchiyan (Talk | contribs) (→‎References)


GFP under the control of constitutive promoter lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


Design Notes

None


Source

The lacI promoter (plac) was obtained from the pAC-EsaR-EsaI plasmid (Shong & Collins, 2013), which was a gift from Cynthia Collins (Addgene plasmid # 47660).

The Green Fluorescence Protein (GFP) gene was obtained from BioBricks Part BBa_E0040 (https://parts.igem.org/Part:BBa_E0040).

References

Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing-dependent gene expression. ACS synthetic biology, 2(10), 568-575.