Part:BBa_K1684002

RBS + spisPink

Pink chromoprotein optimized for E.coli K12 with the standar RBS

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. This protein, expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins.

The protein has a maximum absorption of 560 nm. This characteristic confers a blue shift by at least 14 nanometers in comparison to other known coral chromoproteins, inducing the production of pink color rather than purple appearance.

This version with the standar RBS is codon optimized for the expression in E.coli K12.

Results

In Figure 1 contains samples of parts used for transformation of competent cells the first is composed by RBS (BBa_B0034) and the optimized chromoprotein sequence spisPink for E. coli K12 (BBa_K1684000).The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 3 and Line 4 contain duplicate products of BBa_K1684002 (BBa_B0034 + BBa_K1684000) biobrick, both results match the size of the plasmids, 2800 bp.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680