Composite

Part:BBa_K1684003

Designed by: Juan Jos Quispe Haro   Group: iGEM15_BIOSINT_Mexico   (2015-09-17)
Revision as of 06:25, 18 September 2015 by JuanjoQuispe (Talk | contribs) (→‎Usage and Biology)

RBS + amilGFP

Yellow chromoprotein optimized for E.coli K12 with the standar RBS

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.

This version with the standar RBS is codon optimized for the expression in E.coli K12.

Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680


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