Composite

Part:BBa_K1684004

Designed by: Juan Jos Quispe Haro   Group: iGEM15_BIOSINT_Mexico   (2015-09-17)
Revision as of 06:25, 18 September 2015 by JuanjoQuispe (Talk | contribs) (→‎Usage and Biology)

spisPink + Terminator

Pink chromoprotein optimized for E.coli K12 with the standar Terminator

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. This protein, expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins.

The protein has a maximum absorption of 560 nm. This characteristic confers a blue shift by at least 14 nanometers in comparison to other known coral chromoproteins, inducing the production of pink color rather than purple appearance.

This version with the standar RBS is codon optimized for the expression in E.coli K12.

Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680


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