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Part:BBa_K1645999:Experience

Designed by: Peivand Sadat Mousavi   Group: iGEM15_Waterloo   (2015-09-17)
Revision as of 06:17, 18 September 2015 by Peivandsm (Talk | contribs)

Waterloo-2015-BBa-k1645999.png

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Applications of BBa_K1645999

In order to characterize this part we used Imaging Flow Cytometry to see expression of both Red and Green fluorescent proteins. This part was transformed in DH5-alpha and grown in LB over night. Then they were fixed and diluted 10 times in 10% formalin buffer and then there were places in Imaging FLow Cytometry and they were excited by one laser set at 488nm for optimum range reading. In order to control for signal leakage between channels a compensation was created using each of BBa_I20260 and BBa_J04450 as single colour controls. Then the intensity plot of both colours was created.

The results show that BBa_K1645999 expresses both RFP and GFP in DH5-alpha. Link title

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