Regulatory

Part:BBa_K1628007

Designed by: Xinhao Song   Group: iGEM15_Nankai   (2015-08-04)
Revision as of 06:05, 18 September 2015 by Luckyendin (Talk | contribs)

PamyA

Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), PamyA is the second strongest promoter in our project.

Promoter3 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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