Part:BBa_K1632010

fimB (wild-type)

Fig. 1.

The fim switch is inverted by FimB.The FimB protein inverts the fim switch in the ON-to-OFF direction.

In order to assay the function of our FimB, we added a GFP coding sequence on the downstream of the fim switch.The fim switch[default ON](wild-type)_gfp (BBa_K1632007) emitts fluorescence when expressed, while the fim switch [default OFF](wild-type)_gfp(BBa_K1632008) does not emit florescence when expressed. We also added pBAD/araC on the upstream of fimB.pBAD/araC_fimB (BBa_K1632012) can induce the expression of FimB in the presence of arabinose. We co-transformed a fim switch-gfp and a pBAD/araC_fim recombinase in the E. coli DH5alpha strain. We measured the fluorescence intensity of the cells induced by different concentraions of arabinose.

Fig. 2. The histogram of the samples measured by flow cytometer

From the experimental results, our fimB inverted the fim switch[default ON](wild-type) from ON-to-OFF and the fim switch[defult OFF](wild-type) from OFF-to-ON, depending on the concentration of arabinose.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]