DNA

Part:BBa_K1732013

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)
Revision as of 05:47, 18 September 2015 by Donnalee (Talk | contribs)

J23100-B0034-RFP-His-B0015

His tagged YFP (BBa_K1732025) and strong RBS (BBa_B0034). The J23100 (BBa_J23100) strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.

A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. YFP was measured at (ex/em = 514nm/527nm).


FIntensity.jpg

FQuantitation.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
    Illegal AgeI site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None