Coding

Part:BBa_K1813000

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-08)
Revision as of 05:43, 18 September 2015 by Mvanins (Talk | contribs)

BBa_K1813000 short cch2 GenBank accession no.: (WP_009398853.1)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Background of cch2

Part Description:

Codon optimized coding sequence of CCH2, an enzyme from the ''Bradyrhizobiaceae'' bacterium SG-6C, for E.Coli. This part was used to make our part BBa_K1813001.

Part Origin:

Originally cloned and characterized by Shettigar et al. from Bradyrhizobiaceae bacterial strain SG 6C (1)

Part Function:

The enzyme coded for by ''cch2'' converts 6-Chloronicotinic Acid (6-CNA) to 6-Hydronicotinic acid (6-HNA).

References:

1. Shettigar M, Pearce S, Pandey R, Khan F, Dorrian SJ, et al. (2012) Cloning of a Novel 6-Chloronicotinic Acid Chlorohydrolase from the Newly Isolated 6-Chloronicotinic Acid Mineralizing Bradyrhizobiaceae Strain SG-6C. PLoS ONE 7(11): e51162. doi: 10.1371/journal.pone.0051162

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