Part:BBa_K1859020

Histidine tag and RFP linker [GGSGGS*2] semi-permanent generator

We designed this generator[K1859020] to express long-chain protein with keeping functionality of the protein.

In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．

Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron[BBa_ ] and the 5'side of the intron[BBa_], with those linkers and made them parts. The generator consists of リンカーを結合させたサーキュラーパーツ([BBa_]と[BBa_])、His-RFP without stop codon[BBa_K1332002]、Lacl[]、RBS[]、DT[].

しかし、このgeneratorを導入した大腸菌で発現させたRFPは、蛍光を発するどころか、タンパク質の長鎖化さえされなかった。