Composite

Part:BBa_K1714007

Designed by: Hiroki ONO   Group: iGEM15_HokkaidoU_Japan   (2015-09-17)
Revision as of 03:22, 18 September 2015 by SANBON (Talk | contribs)

pBad/araC-RBS B0034-Ag43 thanatin 4mer as alpha domain-double terminator

Ag43 is a kind of outer membrane protein that is composed of 3 domains. First one is signal peptide that transport Ag43 to outer membrane. Second one is alpha domain that has connection peptide to another Ag43 alpha domain. Last one is beta domain that has beta barrel structure to let the barrel there. This part has thanatin, a kind of antimicrobial peptide 4mer as alpha domain. This thanatin has Asp as the last residue. So if thanatin domain is digested by Asp-N endoproteinase (NEB), this part secrets thanatins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1684
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1966
    Illegal NgoMIV site found at 2476
    Illegal NgoMIV site found at 2497
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1413
    Illegal AgeI site found at 1485
    Illegal AgeI site found at 1557
    Illegal AgeI site found at 1629
    Illegal AgeI site found at 2236
    Illegal AgeI site found at 2650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//collections/antimicrobial
Parameters
None