RNA

Part:BBa_K1859001:Experience

Designed by: Wataru Fukuda   Group: iGEM15_Gifu   (2015-09-07)
Revision as of 03:16, 18 September 2015 by Nomuken (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859001

User Reviews

UNIQ707304ebf820e877-partinfo-00000000-QINU UNIQ707304ebf820e877-partinfo-00000001-QINU

[iGEM gifu 2015] .

We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, we coupled each BBa_K1859004 and [BBa_K1859006] in 3 'sides. In addition,Lacl,RBS and DT conbined to them. When this device was expressed in E.coli, Curcular mRNA including the linker was made. The efficiency that mRNA is circularized changed by sequence of the mRNA. We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.