RNA

Part:BBa_K1859001:Experience

Designed by: Wataru Fukuda   Group: iGEM15_Gifu   (2015-09-07)
Revision as of 03:14, 18 September 2015 by Nomuken (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859001

User Reviews

UNIQ470e0e5970b08f34-partinfo-00000000-QINU UNIQ470e0e5970b08f34-partinfo-00000001-QINU

[iGEM gifu 2015] .

We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, we coupled each BBa_K1859004 and BBa_K1859006 in 3 'sides. In addition,Lacl,RBS and DT conbined to them. When this device was expressed in E.coli, Curcular mRNA including the linker was made. The efficiency that mRNA is circularized changed by sequence of the mRNA. We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.