Coding
Part:BBa_K1689014
Designed by: Wei weijia Group: iGEM15_Peking (2015-09-16)
dCas9-Clact
pSB1C3-6His-dCas9-Clact, C terminal segment of beta-lacatamse(Clact) fused with dCas9, 6×His tag allows for protein purification. β-Lactamase catalyzes the hydrolysis of penicillin to penicillinoic acid, causing current changes which can be detected using A3 electrode system. β-Lactamase could be dissected between Gly196(N termina, Nlactl) and Leu198(C terminal,Clact).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1150
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 3429 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/part_collection/2015
None |