Coding

Part:BBa_K1813004

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-27)
Revision as of 02:40, 18 September 2015 by Rc (Talk | contribs)

CYP6G1

CYP6G1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943


Background of CYP6G1

As an enzyme capable of hydroxylating the 4 and 5 carbons of imidacloprid, it is capable of bestowing a significant protective effect to insects harboring this gene. It has an affinity to 6OH imidacloprid, allowing it to gnerate 4,5 OH imidacloprid, which has an ld50 of over 1 microgram vs 30 nanograms for imidacloprid. Used in conjunction with CYP6CM1vQ, (BBa_K1813003) it can be extremely effective in bestowing imidacloprid resistance to an organism.

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Categories
Parameters
None