Coding

Part:BBa_K1653003

Designed by: Daiki Haraguchi   Group: iGEM15_Nagahama   (2015-05-31)
Revision as of 02:17, 18 September 2015 by Naoki (Talk | contribs)

ispA

ispA(from Escherichia coli JM109)

ispA encodes not only geranyl diphosphate synthase but also farnesyl diphosphate synthase.

Farnesyl diphosphate synthase can utilize both dimethylallyl and geranyl diphosphates as substrates, generating geranyl and farnesyl diphosphate, respectively. Therefore the enzyme can catalyze two sequential reactions in the polyisoprenoid biosynthetic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 867
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None