Composite

Part:BBa_K1806002

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM15_AUC_TURKEY   (2015-09-17)
Revision as of 00:04, 18 September 2015 by Registry (Talk | contribs)

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T7 lacO pelB RFP 6xHis

The part has been designed as a backbone part to integrate the T7 lacO pelB + 6xHis system to a targeted sequence. The RFP has BamH1 and Xho1 restriction sites to be cut out and replaced by the targeted sequence. T7 lacO allows the system to be inducible by iPTG and the pelB signal peptide allows the cells to excrete the translated proteins placed in the following sequence. The 6xHis is present for protein analysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2
    Illegal BamHI site found at 196
    Illegal XhoI site found at 881
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 163
    Illegal AgeI site found at 757
    Illegal AgeI site found at 869
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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