Not Released
Sample In stock
Experience: Issues
Not Used
Get This Part
Regulatory

Part:BBa_K1766001

Designed by: Sarah Wideman   Group: iGEM15_Stockholm   (2015-09-05)
Revision as of 23:40, 17 September 2015 by Sarahwideman (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

MicF Target


The micF target stems from the 5’ untranslated region of the ompF (outer membrane porin F) gene in E. coli. It is the bind site for micF, a non-coding regulatory RNA. MicF prevents translation of ompF when it binds to the micF target [1].

The micF target is intended to be fused to a protein of interest. It should then be possible to down-regulate protein expression using micF (BBa_K176600).

BBa_K1766001 consists of the micF target and Anderson promoter BBa_J23101. The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12


[edit]
Categories
Parameters
None