Part:BBa_K1766001

MicF Target

The micF target stems from the 5’ untranslated region of the ompF (outer membrane porin F) gene in E. coli. It is the bind site for micF, a non-coding regulatory RNA. MicF prevents translation of ompF when it binds to the micF target [1].

BBa_K1766001 consists of the micF target and Anderson promoter BBa_J23101.

The micF target is intended to be fused to a protein of interest. It should then be possible to down-regulate protein expression using micF (BBa_K176600).

The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12