Composite

Part:BBa_K1707026

Designed by: Sylvie Lautru, Audrey Moatti   Group: iGEM15_Paris_Saclay   (2015-09-14)
Revision as of 23:26, 17 September 2015 by SylvieL (Talk | contribs) (→‎Usage and Biology)

CFP-ssRA under the control of J23101

This part was constructed to have a positive control for the expression of CFP-ssRA (under the control of a strong promoter).


Usage and Biology

We characterized this part using fluorescence microscopy.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170726

Paris Saclay K1707026 w1POL.png Differential interference contrast Paris Saclay K1707026 w2Lumencor CFP 434-17.png excitation with led 437 nm/17nm (CFP), 100 ms of exposure


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None