RNA
MicF

Part:BBa_K1766000

Designed by: Sarah Wideman   Group: iGEM15_Stockholm   (2015-08-26)
Revision as of 23:22, 17 September 2015 by Sarahwideman (Talk | contribs)

MicF non-coding regulatory RNA


MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].

BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.

Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

[edit]
Categories
//chassis/prokaryote/ecoli
//regulation/negative
Parameters
biologyEscherichia coli