Part:BBa_K1758343

MerR activator under constitutive promoter and induceble merT promoter with 5 UTR -sfGFP const. prom + merR+merT-UTR-sfGFP mercury sensor achtivator under the control of a constitutive promoter with mercury induceble promoter and 5´untranslated region which increses the output of the sfGFP which is used for detection.

Usage and Biology

For our sensor, we use parts ( BBa_K346002 and BBa_K346001) of the mercury sensor constructed by iGEM team Peking 2010. These Parts consist of the Mer operon from Shigella flexneri R100 plasmid Tn21, a mercury dependent operon. The expression of the Mer operon is regulated by the activator MerR. The MeR transcription however is regulated by itself. Mercury can bind to the C-terminal located cysteines and generates a conformal change to activate the expression (N.L. Brown et al.2003). Our mercury Sensor contains MerR, which is under control of a constitutive promoter and specific promoter MerT. sfGFP protein is used as measuring output signal and it´s transcription is controlled by the 5` untranslated region, which enhances the following reporter protein sfGFP .

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 462
  Illegal NheI site found at 485
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 626