Coding

Part:BBa_K1692002

Designed by: Daniel Kunin   Group: iGEM15_Stanford-Brown   (2015-08-03)
Revision as of 23:02, 17 September 2015 by Daniel.kunin (Talk | contribs)

codon optimized FDC with T7 promoter and Flag Tag

Introduction

Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.

Styrene synthesis pathwayThis is where we write


Styrene synthesis enzymesThis is where we write


Background

SDS PAGE Gel of Enzymes for Styrene SynthesisThis is a SDS PAGE gel with purified PAL, FDC and UbiX protein. We ran a Mark 12 protein ladder to verify that our proteins were the correct molecular weight.


Styrene AbsorbanceThis is where we write


Flavin Mononucleotide AbsorbanceThis is where we write


Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2015
Parameters
None