Coding
MorA

Part:BBa_K1615000:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-27)
Revision as of 22:37, 17 September 2015 by Brooke (Talk | contribs) (→‎Source)

Morphine-6-dehydrogenase RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 840
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To make this gene standardised it was codon optimised for the chassis Esherichia coli as well as making it RFC25 compatible which required removing all illegal restriction sites in the gene sequence.


Source

Genomic sequence from Pseudomonas putida.

References