Coding

Part:BBa_K1585200

Designed by: Carolin Grütering   Group: iGEM15_Aachen   (2015-08-24)
Revision as of 22:28, 17 September 2015 by Tschwanemann (Talk | contribs)

Mdh2

Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NAD+ dependent alcohol dehydrogenase that converts methanol to formaldehyde.


Catalysed reaction by Mdh
Methanol + NAD → Formaldehyde + NADH + H+.


Usage and Biology

The Characterization was done with RBS B0034 and is documented here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 577
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None