Coding

Part:BBa_K1692004

Designed by: Daniel Kunin   Group: iGEM15_Stanford-Brown   (2015-08-03)
Revision as of 22:28, 17 September 2015 by Daniel.kunin (Talk | contribs)

codon optimized PAL with T7 promoter and Flag Tag

Introduction

Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.

Styrene synthesis pathwayThis is where we write


Styrene synthesis enzymesThis is where we write


Background

SDS PAGE Gel of Enzymes for Styrene SynthesisThis is where we write



TitleThis is where we write


PAL FunctionalityThis is where we write


Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1533
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2015
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None