Device

Part:BBa_K1587007

Designed by: Alexandre Le Scornet   Group: iGEM15_Toulouse   (2015-09-15)
Revision as of 21:41, 17 September 2015 by JohnSmith (Talk | contribs)

Formate production

Strong RBS (BBa_1006) and formate producing enzyme pyruvate formate-lyase 1 pflB and pyruvate formate-lyase 1-activating enzyme pflA.

TLSE For.jpg
Figure 1: Genetic construct for formate production


TLSE ForMetabo.jpg
Figure 2: Metabolic pathway to produce formate via glucose using BBa_K1587007 in E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 405
    Illegal BamHI site found at 2807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 514
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2995


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Categories
Parameters
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