Composite

Part:BBa_K1799022

Designed by: Will Shindel, Michael Flanagan   Group: iGEM15_Genspace   (2015-09-17)
Revision as of 20:39, 17 September 2015 by Registry (Talk | contribs)

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IPTG-Repressible RFP Generator

Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system. The device consists of two parts, separated by a terminator.

Part 1: LsrR open reading frame, under the control of the Lac promoter and a medium RBS. Part 2: RFP under the control of pLsrA and a medium RBS.

When cultured in a LacI- strain or in the presence of IPTG, LsrR is produced, repressing pLsrA and preventing expression of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1902
    Illegal AgeI site found at 2014
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None