RNA

Part:BBa_K1797007

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)
Revision as of 18:42, 17 September 2015 by Registry (Talk | contribs)

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tet promoter-sgRNA-terminator

This part is modified from BBa_K1797005. We added promoter and terminator before and after the sgRNA for the convenience of users. Users can change the sequence of sgRNA(except for the sgRNA scaffold) for their own purpose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 262
    Illegal BsaI site found at 440
    Illegal SapI site found at 84
    Illegal SapI.rc site found at 73


[edit]
Categories
Parameters
None