Coding

Part:BBa_K1797006

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)
Revision as of 18:16, 17 September 2015 by Registry (Talk | contribs)

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dCas9

CRISPR Cas9 is a very popular gene editing system. This part includes an engineered dCas9 which is still able to bind to the DNA under the guidance of gRNA. However, it loses its catalytic activity. Actually, dCas9 has a very promising future. For example, We can use dCas9 to block the recruitment of transcription machine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None