Coding
Holin-rev

Part:BBa_K1616007

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-17)
Revision as of 16:17, 17 September 2015 by Chesne j (Talk | contribs)

Holin reversed



Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)

This part is the reversed sequence of the holin (BBa_K112805); thus this part can be used with a reversed endolysin (BBa_K1616006), reversed promoter, reversed RBS and reversed terminator.

This part is the reverses sequence of the BBa_K112805 (Holin).




Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The illegal sites have been checked


Source

This part is the reverses sequence of the BBa_K112805 (Holin).

References

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Categories
Parameters
None