Plasmid_Backbone

Part:BBa_K1680016

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 14:46, 17 September 2015 by Registry (Talk | contribs)

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pRS316 yeast shuttle vector with Biobrick MCS

A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2040
    Illegal XbaI site found at 2025
    Illegal SpeI site found at 2017
    Illegal PstI site found at 2003
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2040
    Illegal SpeI site found at 2017
    Illegal PstI site found at 2003
    Illegal NotI site found at 2008
    Illegal NotI site found at 2032
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2040
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2040
    Illegal XbaI site found at 2025
    Illegal SpeI site found at 2017
    Illegal PstI site found at 2003
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2040
    Illegal XbaI site found at 2025
    Illegal SpeI site found at 2017
    Illegal PstI site found at 2003
    Illegal NgoMIV site found at 1668
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1079
    Illegal BsaI.rc site found at 3414
    Illegal SapI site found at 2331
    Illegal SapI site found at 4435
    Illegal SapI.rc site found at 926


[edit]
Categories
//chassis/eukaryote/yeast
//dna
//plasmid
//plasmidbackbone
//plasmidbackbone/copynumber/low
Parameters
None