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Coding

Part:BBa_K1583112

Designed by: Stefan Robert Marsden, Max van 't Hof, Samantha Basalo Vazquez   Group: iGEM15_TU_Delft   (2015-09-15)
Revision as of 14:46, 17 September 2015 by SMarsden (Talk | contribs)

pRha + CsgA & GFP in same operon

CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. The aggregation to the nanowire ('curli') is induced by the membrane protein CsgB. CsgC, CsgE, CsgF and CsgG act as chaperones for CsgA during translation and export CsgA to the extracellular space. This part was designed to measure intracellular expression rates of CsgA coupled to fluorescence (GFP) by cloning the biobrick I13504 into the same operon which is under control of the Rhamnose promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1308


[edit]
Categories
//awards/part_collection/2015
Parameters
None