Part:BBa_K1587004

Butyrate synthesis pathway regulated by constitutive promoter p(Bla)

Constitutive promoter p(Bla). Crotonyl-coA reductase ccr coding region. 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly NMR analysis did not yield a significant over production of butyrate in E. coli.

Figure 1: Genetic construct to produce butyrate via glucose

Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 36
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1717
  Illegal BamHI site found at 3634
  Illegal XhoI site found at 1407
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 332
  Illegal AgeI site found at 1254
  Illegal AgeI site found at 2900
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1752
  Illegal BsaI.rc site found at 2169
  Illegal BsaI.rc site found at 3237
  Illegal SapI site found at 123
  Illegal SapI.rc site found at 3249