Part:BBa_K1696007

RcfB

The trans-acting protein RcfB, which belongs to the Crp-Fnr family of transcription regulators, is involved in basal activity of P170 and is also essential for pH induction. To our knowledge, no Crp-Fnr protein is known to respond to an acid signal, so it is an remarkable finding that RcfB is activated when the cells are exposed to acid environments. The protein RcfB contains conserved glycine residues, activating regions 1 and 3, D α-helix, and C-terminal helix-turn-helix (HTH) DNA binding motif, which, upon activation, bind a DNA recognition motif within a promoter region and activates transcription.

Reference

Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]