Coding

Part:BBa_K1680018

Designed by: Nicolai von Kügelgen   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 14:11, 17 September 2015 by Registry (Talk | contribs)

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Cre-Dronpa fusion

Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 458


[edit]
Categories
//cds
//cds/reporter/chromoprotein
//cds/reporter/gfp
//chassis/eukaryote/yeast
//function/recombination/cre
//function/reporter/fluorescence
Parameters
colorGreen