Part:BBa_K1696005

P170 Promoter

P170 is upregulated at low pH (6.0~6.5) during the transition to stationary phase. The minimal DNA region required for both promoter activity and pH regulation was mapped to a 51 bp fragment located 7 bp upstream of the transcriptional start site. This fragment lacked the consensus -35 promoter region, but it contained an ‘extended’ -10 promoter region[1]. Besides, a novel 14 bp regulatory DNA region centered at around -41.5 and composed of three tetra nucleotide sequences, boxes A, C and D. Boxes A and C contribute to P170 activity, whereas box D and the position of boxes ACD (renamed ACiD-box) are essential to P170 activity and acid response[2].

Reference

[1] Madsen S M, Arnau J, Vrang A, et al. Molecular characterization of the pH-inducible and growth phase-dependent promoter P170 of Lactococcus lactis.[J]. Molecular Microbiology, 1999, 32(1):75-87. [2] Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]