Coding
Part:BBa_K1680017
Designed by: Nicolai von Kügelgen Group: iGEM15_Tuebingen (2015-09-17)
Cre-Dronpa Fusion
Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with intermediate 6 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1327
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 449
Illegal SapI site found at 1046
[edit]
Categories
Parameters
//cds
//cds/reporter/chromoprotein
//cds/reporter/gfp
//chassis/eukaryote/yeast
//function/recombination/cre
//function/reporter/fluorescence
//cds/reporter/chromoprotein
//cds/reporter/gfp
//chassis/eukaryote/yeast
//function/recombination/cre
//function/reporter/fluorescence
color | Green |