Coding
Part:BBa_K1796007
Designed by: Nannan Xie Group: iGEM15_SCU_China (2015-09-17)
nifB from Paenibacillus sp. WLY78
essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1156
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1156
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1156
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1156
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1156
Illegal AgeI site found at 129
Illegal AgeI site found at 1089
Illegal AgeI site found at 1444 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1261
[edit]
Categories
Parameters
protein |