Composite

Part:BBa_K1824003

Designed by: Wenbo Xu, Hao Wu   Group: iGEM15_XJTLU-CHINA   (2015-09-05)
Revision as of 13:03, 17 September 2015 by Yumenwh (Talk | contribs) (→‎Characterization)

pBAD + RNA Thermometer A1

This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer A1 BBa_K1824557 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.


A1 RNA Thermometer.jpg
Figure 1: Possible secondary structure of RNAT A1.


Characterization

PBad.jpg
Figure 2: The fluorescence of pBad+A1 with different dilution ratio at 30℃ and 37℃(Control and Arabinose).

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