Translational_Unit

Part:BBa_K1632020

Designed by: Jun kawamura   Group: iGEM15_Tokyo_Tech   (2015-08-30)
Revision as of 12:39, 17 September 2015 by JunKawamura (Talk | contribs)

rbs_CmR(ssrA degradation tag)

At the first stage of wet experiment, we used the cells which have the plasmid which is Pcon_rhlR_TT_Plux_rbs_cmR to characterize the function of rbs_cmR(BBa_K395160 by iGEM10_Tokyo_Tech). But the cells showed active growth even in the absence of AHL (Fig. 1.The cells growth with Cm)

Fig. 1. The cells growth with Cm

From the results of this experiment, initially designed circuits showed leaky expression of CmR. So we inserted an ssrA degradation tag to the C-terminal of CmR.

For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None