Composite
2 x FYVE

Part:BBa_K1769000:Experience

Designed by: Ho-Tsang Tsai   Group: iGEM15_NYMU-Taipei   (2015-09-14)
Revision as of 10:45, 17 September 2015 by MichaelT (Talk | contribs) (→‎Applications of BBa_K1769000)


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Applications of BBa_K1769000

Results

We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.

The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10-6M wortmannin, dimeric FYVE redistributed in cytoplasm.


User Reviews

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