Composite
KaiABC
Part:BBa_K1745001:Design
Designed by: Mansi Chandra Group: iGEM15_UChicago (2015-09-17)
KaiABC Oscillator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
Illegal NheI site found at 3037 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 410
Illegal XhoI site found at 477 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2945
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 540
Design Notes
In vitro investigations of this modular system revealed that the robustness of oscillations was sensitive to stoichiometric ratios of KaiA to KaiC (Nakajima, Science 2015). To experiment with these stoichiometric ratios, KaiB and KaiC expression are driven under a constitutive promoter (Part J23100) while KaiA expression is driven under a L-rhamnose inducible promoter (Part K914003).
Source
The KaiABC circadian oscillator is endogenous to the cynanobacterial species Synechococcus elongatus.