Composite
KaiABC

Part:BBa_K1745001:Design

Designed by: Mansi Chandra   Group: iGEM15_UChicago   (2015-09-17)
Revision as of 07:40, 17 September 2015 by Registry (Talk | contribs)

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KaiABC Oscillator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
    Illegal NheI site found at 3037
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 410
    Illegal XhoI site found at 477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 540


Design Notes

In vitro investigations of this modular system revealed that the robustness of oscillations was sensitive to stoichiometric ratios of KaiA to KaiC (Nakajima, Science 2015). To experiment with these stoichiometric ratios, KaiB and KaiC expression are driven under a constitutive promoter (Part J23100) while KaiA expression is driven under a L-rhamnose inducible promoter (Part K914003).


Source

The KaiABC circadian oscillator is endogenous to the cynanobacterial species Synechococcus elongatus.

References