Regulatory

Part:BBa_K1592020:Design

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-09)
Revision as of 05:41, 17 September 2015 by WangjieLiu (Talk | contribs) (→‎Design Notes)

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Ptrp mutant1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the trp promoter has two illegal restriction enzyme sites (SpeI),we designed the point mutantion to improve it.

Source

Cloned from BBa_K588001 and mutated by overlap extension PCR.

References