Coding
Si-tag1+3

Part:BBa_K1592012:Design

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-07)
Revision as of 04:55, 17 September 2015 by WangjieLiu (Talk | contribs) (→‎Design Notes)

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LIP prepro + E. coli ribosomal protein L2 (1-60,203-273aa) + YLcwp3 Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
    Illegal XhoI site found at 572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 593


Design Notes

In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2(1-60,203-273aa), GS linker and YLcwp3

Source

E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.

References